| 1 | Mirror Show Management | Tammy Wilkes | Services |
| 2 | Henderson Ford/MV-1 of Rochester | Randy Henderson | Retail |
| 3 | Bob Johnson Chevrolet | Greg Stahl | Services |
| 4 | Dixon Schwabl | Lauren Dixon | Services |
| 6 | Emerge | Leo Linder | Services |
| 7 | Waste Harmonics, LLC | Michael Hess | Services |
| 8 | ConServe | Mark Davitt | Services |
| 9 | ClickSpark, LLC | Thomas Tortora | Services |
| 10 | Rochester Precision Optics LLC | Dane Hileman | Manufacturing |
| 11 | SunCommon | Kevin Schulte | Services |
| 12 | Angels In Your Home | | |
| 13 | Distributed Technology Group | | Services |
| 14 | PKG Equipment Inc. | Carla Pontarelli | Manufacturing |
| 15 | Steve General Contractor, Inc. | | Services |
| 16 | Custom Courier Solutions, Inc. | Daniel Ayer | Services |
| 17 | Taylor, the Builders | Karl Schuler | Services |
| 18 | Leonard's Express | | Services |
| 19 | Sutherland Global Services | Dilip Vellodi | Services |
| 20 | LSI Solutions, Inc. | Jude Sauer | Manufacturing |
| 21 | Calvary Automation Systems, Inc. | Mark Chaney | Manufacturing |
| 22 | Christian Flooring, Inc. | | Services |
| 23 | Avani Technology Solutions Inc. | Sameer Penakalapati | Services |
| 24 | Liberty Pumps, Inc. | | |
| 25 | Broadstone Real Estate, LLC | Amy Tait | Services |
| 26 | The Bonadio Group | Thomas Bonadio | Financial |
| 27 | Soleo | Dan Gallagher | Services |
| 28 | Classified 'Yellow Page-One' | | |
| 29 | Wellington Steele & Associates | Edward Leva | Services |
| 30 | ProNexus, LLC | Rafael Vidal | Services |
| 31 | Xelic, Inc. | | Services |
| 32 | Pioneer Millworks/New Energy Works of Rochester | Jennifer Young | Services |
| 33 | LaBella Associates | Robert Healy | Services |
| 34 | Operation Brain Freeze, LLC DBA: Dairy Queen | Mark Goldberg | Retail |
| 35 | D4 | Martha MacPherson | Services |
| 36 | Cheribundi | | |
| 37 | Northern Air Systems, Inc. | Joe Denninger | Manufacturing |
| 38 | Apollo Optical Systems, Inc. | | Services |
| 39 | P3 Systems, Inc. | Frank Pecora | Services |
| 40 | MaeTec Power, Inc. | | Services |
| 41 | C.M. Armitage | | |
| 42 | id Signsystems Inc. | Paul Dudley | Services |
| 43 | Century Mold Co. Inc. | | |
| 44 | Power Management Co., LLC | John Burt | Services |
| 45 | Abtex Corporation | D. Mark Fultz | Manufacturing |
| 46 | Triad Network Technologies | Peter Sweltz | Services |
| 47 | Keller Williams-Scripa Group | | |
| 48 | Genesee Regional Bank | Philip Pecora | Financial |
| 49 | Wright Beverage Distributing | Claude Wright | Wholesale/Distribution |
| 50 | MediSked, LLC | Douglas Golub | Services |
| 51 | Admar Supply Co., Inc. | Joel DiMarco | Services |
| 52 | 3G Packaging, Inc. | Vincent Stango | Services |
| 53 | Ramar Steel Inc. | | Manufacturing |
| 54 | VP Supply Corp. | Gary Curwin | Wholesale/Distribution |
| 55 | Birchcrest Tree and Landscape, Inc. | | Services |
| 56 | New York Technology Partners, Inc. | | Services |
| 57 | Wegmans Food Markets, Inc. | Daniel Wegman | Retail |
| 58 | Hammer Packaging Corp. | James Hammer | Manufacturing |
| 59 | Rollison Construction Sales, LLC | | Wholesale/Distribution |
| 60 | Capstone Information Technologies Inc. | Sitima Fowler | Services |
| 61 | American Rentals, LLC dba The Duke Company | David Terry | Services |
| 62 | Isaac Heating & Air Conditioning, Inc. | Raymond Isaac | Services |
| 63 | Butler/Till Media Services, Inc. | Kimberly Jones | Services |
| 64 | Conifer Realty, LLC | Timothy Fournier | Services |
| 65 | Klein Steel Service Inc. | Todd Zyra | Manufacturing |
| 66 | Patriot Towers, Inc. | Richard Schickler | Services |
| 67 | Innovative Solutions | Justin Copie | Services |
| 68 | 2TouchPOS | | Services |
| 69 | Alesco Advisors, LLC | | Financial |
| 70 | UniLink Inc. | Lou Fuchs | Services |
| 71 | OptiPro Systems | | Manufacturing |
| 72 | Nu-Look Collision | Todd Zigrossi | Services |
| 73 | Special Care Systems, LLC | Ann Kowal | Health Care |
| 74 | Dorschel Lexus | Richard Dorschel | Retail |
| 75 | Gardner Plus Architects, PLLC | | |
| 76 | Steel Tech Fabricators | | |
| 77 | The Pike Company Inc. | Rufus Judson | Services |
| 78 | Martino Flynn, LLC | Kelly Goforth | Services |
| 79 | Systems Management Planning | Eric Rorapaugh | Services |
| 80 | eHealth Technologies | Ken Rosenfeld | Health Care |
| 81 | Hamilton Stern Construction | Justin Hamilton | Services |
| 82 | Omega Tool Measuring Machines Inc. | Michael Nuccitelli | Manufacturing |
| 83 | Spex Precision Machine | Michael Nolan | Manufacturing |
| 84 | CaterTrax | Rich Rund | Services |
| 85 | DeCarolis Truck Rental Inc. | Paul DeCarolis | Wholesale/Distribution |
| 86 | Brite | Justin Smith | Services |
| 87 | American Packaging Corp. | Peter Schottland | Manufacturing |
| 88 | Classic Automation LLC | Fritz Ruebeck | Services |
| 89 | Mercury Print Productions, Inc. | John Place | Manufacturing |
| 90 | Bergmann Associates | Thomas Mitchell | Services |
| 91 | Harris Beach PLLC | James Spitz | Services |
| 92 | HR Works, Inc. | Candace Walters | Services |
| 93 | New York Patriot Construction, Inc. (DBA Patriot Construction) | Jan Brewster | Services |
| 94 | Davidson Fink LLP | Robert Galbraith | Services |
| 95 | Sheridan Brothers Moving Inc. | Mike Sheridan | Services |
| 96 | Biomaxx, Inc. | Dan Wetzel | Manufacturing |
| 97 | Tender Loving Family Care, Inc. | Annika D'Andrea | Health Care |
| 98 | Redman Construction, Inc. | | Services |
| 99 | Van Zile Travel Service | Rebecca Mineo | Services |
| 100 | Upstate Door, Inc. | Rob Fontaine | Manufacturing |